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| Registros recuperados: 101 | |
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| Benjamin,Sailas; Pandey,Ashok. |
| Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40ºC and pH 7-8.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida rugosa; Lipases; Solid cultures; Purification; Characterization. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500002 |
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| Zappa, Sebastien; Rolland, Jean-luc; Flament, Didier; Gueguen, Yannick; Boudrant, Joseph; Dietrich, Jacques. |
| This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequence showed conservation of the active site and structural elements of the E. coli AP. The recombinant AP was purified and characterized. Monomeric and homodimeric active forms were detected, with a monomer molecular mass of 54 kDa. Apparent optimum pH and temperature were estimated at 11.0 and 70 degreesC, respectively. Thus far, P. abyssi AP has been demonstrated to be the most thermostable AP, with half-lives at 100 and 105 degreesC of 18 and 5 h, respectively.... |
| Tipo: Text |
Palavras-chave: Escherichia coli; Characterization; Isolation; Gene; Alkaline phosphatase; Pyrococcus abyssi; Archaeon. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-1268.pdf |
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| Benjamin,Sailas; Pandey,Ashok. |
| Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40°C and pH 7-8.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida rugosa; Lipases; Solid cultures; Purification; Characterization. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200016 |
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| Vasconcellos,Luciana Pimentel de Mello Klocker; Tambasco-Talhari,Daniella; Pereira,Andréa Pozzi; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida. |
| Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the kappa-casein - HinfI (CSN3 - HinfI), beta-lactoglobulin - HaeIII (LGB - HaeIII) and growth hormone AluI (GH- AluI) genes, as well as four microsatellites (TEXAN15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated. The values for these parameters of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aberdeen Angus; Bovine; DNA; Characterization; RFLP; Microsatellite. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200005 |
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| Higuti,lma Hiroko; Grande,Simone Wichert; Sacco,Roberta; Nascimento,Aguinaldo José do. |
| One hundred and twenty five soil samples were collected from the regions of roots of corn, cassava, potato, bean, sugar cane, soya, and pumpkin. From these, 75 strains were isolated that produced a yellowish halo surrounding the colonies, due to a phenolphtalein-cyclodextrin (CD) complex, and these were selected as alkalophilic CGTase-producing bacteria. All the 75 strains were identified as Bacillus firmus by microscopy and biochemical tests. The activity of the CGTase's varied from 2² to 2(10) dilutions,when assayed by CD-trichloroethylene (TCE)-complex precipitation. Strain 31 that produced the enzyme at the higher level was selected, and its enzyme was partially purified by starch adsorption (x 17) in a yield of 51%. Maximum enzyme activity occurred at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Characterization. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200007 |
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| Arns,C.W.; Campalans,J.; Costa,S.C.B.; Domingues,H.G.; D'Arce,R.C.F.; Almeida,R.S.; Coswig,L.T.. |
| This paper presents the first isolation of bovine respiratory syncytial virus in Brazil and its physicochemical, morphological and molecular characterization. The virus was isolated from 33 samples of nasotracheal secretions, successively inoculated into a Madin-Darby bovine kidney cell culture, which was characterized by physicochemical tests and morphological observation by electron microscopy. The Brazilian sample is an RNA pleomorphic, enveloped, thermolabile and non-hemagglutinating spicular virus. Reverse transcription, followed by nested polymerase chain reaction (nRT-PCR) assay was carried out using oligonucleotides B1, B2A, B3 and B4 for the fusion proteins (F) and B5A, B6A, B7A and B8 for the attachment protein (G). The nRT-PCR-F amplified a... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bovine respiratory syncytial virus; BRSV; Virus isolation; Characterization; Nested RT-PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200008 |
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| Otero,Laura; Ducasse,Daniel; Miller,Robert N. G.. |
| Using PCR-based assays with specific primers for amplification of the ribosomal DNA intergenic spacer region (IGS) and a portion of the mitochondrial DNA small subunit ribosomal RNA gene (mtDNA SSU rRNA), the genetic variability among Verticillium dahliae isolates from olive (Olea europaea) and other host species from Argentina and Brazil was estimated. The derived UPGMA-generated phenograms based upon the restriction fingerprinting data of rDNA IGS products revealed genetic differences, correlating with the host of origin. Isolates infecting olive genetically distinct from those from cocoa (Theobroma cacao) and sunflower (Helianthus annuus). Digestion of mitochondrial DNA SSU rRNA PCR products revealed less variability, distinguishing only one isolate... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Olea europaea; Molecular markers; Characterization. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000400015 |
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| Souza,Vanessa Ribeiro de; Silva,Ana C. G.; Pinotti,Laura Marina; Araújo,Heloísa Sobreiro Selistre; Giordano,Raquel de Lima Camargo. |
| The purpose of this work was to characterize the enzyme penicillin G acylase (PGA) produced by Bacillus megaterium. Purification of the enzyme by ultra/diafiltration did not allow the detection of the PGA band by SDS-PAGE electrophoresis due to the high content of remaining proteins. However, using the DNA of the microorganism, it was possible to replicate the genes of the two B. megaterium PGA reported in literature, showing that the enzyme consisted of two sub-units, having 245 and 537 amino acids each and an average molecular mass of 26950 and 59070 Da, respectively. The parameters studied were: 1) the influence of temperature in the 25-60(0)C range, 2) pH in the 5-10 range and 3) substrate concentration, this was tested to obtain results on the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Penicillin G acylase; Bacillus megaterium; Characterization. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400013 |
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| Melgarejo,Luz Marina; Vega,Nohora; Pérez,Gerardo. |
| Two lectins were isolated from Canavalia ensiformis and Dioclea grandiflora seeds. Gel filtration produced a fraction corresponding to Con A or D. grandiflora lectin while erythroagglutination assays revealed a distinct fraction presenting a lectin that agglutinates human red blood cells (RBCs) but not rabbit RBCs. Hydrophobic interaction chromatography showed that the latter fraction yielded a protein that readily agglutinates human erythrocytes; the lectin was also purified by affinity chromatography on Lac-Sepharose showing similar properties to that of the Phenyl-Sepharose-purified lectin. Despite minor differences (carbohydrate content or A1%1cm), the two lectins showed similar molecular properties in that they consisted of two non-covalently linked... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diocleinae; Leguminoseae; Characterization; Lectins. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000300006 |
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| González Cerón, Fernando. |
| El presente estudio tuvo como objetivo caracterizar la infestación por garrapatas en un hato experimental de ganado Criollo Lechero Tropical (CLT) ubicado en la región central del estado de Veracruz, México; la información generada permitiría iniciar el registro sistemático de la infestación causada por éstos ectoparásitos para considerarla como una posible característica a ser incluida en un programa de mejora genética a mediano plazo. A 28 vaquillas (655±35 días de edad) y 16 toretes (741±89 días de edad) CLT se les retiraron del cuerpo todas las garrapatas saturadas (≥4.5 mm) considerando 18 regiones corporales para identificar la especie a la que pertenecían, ubicar las regiones corporales donde se localizaban, determinar la carga parasitaria y estimar... |
| Tipo: Tesis |
Palavras-chave: Criollo lechero tropical; Garrapata; Caracterización; Maestría; Ganadería; Tropical milking criollo; Tick; Characterization. |
| Ano: 2007 |
URL: http://hdl.handle.net/10521/1623 |
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| Hernández Flores, Martín. |
| La investigación ha privilegiado la identificación y caracterización de un bien histórico de gran importancia patrimonial y valor presente, que se manifiesta como factor fundamental a través de un conjunto de poblaciones de maíces nativos -conocidos localmente como criollos-, al interior de una amplia diversidad y multifuncionalidad de sistemas productivos, en la región de la Costa Chica de Guerrero. La tendencia de la modernización de la agricultura durante los últimos años, demuestra que en la región se ha excluido sistemáticamente el valor presente de los recursos locales, los cuales indudablemente forman parte de las lógicas de funcionamiento de los sistemas de producción campesinos e indígenas. En particular, ha sido y es notorio el desinterés... |
| Tipo: Tesis |
Palavras-chave: Tipos de maíz criollo; Sistemas de cultivo; Caracterización; Maestría; Desarrollo Rural; Types of creole maize; Systems of culture; Characterization. |
| Ano: 2007 |
URL: http://hdl.handle.net/10521/1557 |
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| Registros recuperados: 101 | |
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